You will find 21 G- 1.5 inch needle & 21 G - 1 inch needle in the kit. These two needle serve two different functions. The long needle is for loading the sample into the device at the bottom. Short needle is for aspirating the top fraction of swim-up media after 30 -45mins post loading. Short needle allows you to take 0.8-1 ml of top fraction of media with ease.